Note that some restriction enzymes are not fully inactivated by heat treatment.

Samples can be stored for up to a week at 4&176; C (refrigerator) or can be stored at -20&176; C (freezer) for up to 5 years.

For some downstream applications it is necessary to heat-inactivate the enzyme after digestion.

CutSmartTM Buffer) - Plasmid DNA Procedure - Prepare a 25 ul reaction Component Amount Restriction Enonuclease 1 ul for each used enzyme CutSmartTM Buffer 3 ul Nuclease-free water Up to 25 ul - Incubation for 1h at 37C (or at different. Dec 15, 2021 &0183; HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA.

This product can be used in the following applications Fast Cloning Accelerate your cloning.

- 10 l of 10x restriction buffer - B l of sterile MQ water.

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Time-Saver qualified enzymes can cut substrate DNA in 5-15 minutes and safely digest overnight.

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2. A restriction enzyme may lose activity due to improper storage or handling.

. Time-Saver qualified enzymes can cut substrate DNA in 5-15 minutes and safely digest overnight.

A complete system of restriction enzymes and DNA-modifying enzymesfor beautifully simple cloning.
Keep all the stock solutions on ice.
unless it is wrapped around with parafilm.

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Time-Saver qualified enzymes can cut substrate DNA in 5-15 minutes and safely digest overnight.

keep it in 4 degree. Procedure. .

calculate how much water to add to the digest; this is (100-10-2-A) call this B. Label your tube(s). Quick Guide For Restriction Digestion and Analysis DNA RB Ice L P EH Tap Centrifuge Water bath. ApaI has 100 activity at 37&176;C, however the half-life of this enzyme at 37&176;C is only 30 minutes. .

25C.

For each restriction enzyme, we report the minimum number of units (1. .

Pro-Tip To determine which restriction enzymes will cut your DNA sequence (and where they will cut), use a sequence analysis program such as.

If two different incubation temperatures are necessary, choose the optimal reaction buffer and set up reaction accordingly.

CutSmartTM Buffer) - Plasmid DNA Procedure - Prepare a 25 ul reaction Component Amount Restriction Enonuclease 1 ul for each used enzyme CutSmartTM Buffer 3 ul Nuclease-free water Up to 25 ul - Incubation for 1h at 37C (or at different.

Restriction enzymes are typically inactivated.

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